Environmental Services - Aquatic Toxicity Monitoring (or WET):
James R. Reed and Associates is a Virginia Department of Environmental Quality (DEQ) approved environmental testing laboratory with over 30 years of experience offering Aquatic Toxicity Testing. Our goal is to help NPDES permittees maintain compliance by providing reliable testing results with a convenient testing schedule.

Testing Convenience:
• We pre-schedule your toxicity testing according to your NPDES permit
• We provide sample pickup and free sample kits
• We receive your toxicity samples 7 days a week with your advanced notice

Customer Service:
• We provide free test quotation based on your permit
• Experienced staff that are able to answer your questions anytime within our business hours
• Immediate test result information provided by phone

Toxicity Testing Services (or WET) for NPDES discharge permits

Acute Test:
Daphnia pulex
Ceriodaphnia dubia
Pimephales promelas
Americamysis bahia
Cyprinodon variegatus

Chronic Test:
Ceriodaphnia dubia
Pimephales promelas
Americamysis bahia
Cyprinodon variegates

QA/QC program:
• Our toxicity testing procedures follow EPA approved methods and Virginia DEQ guidance
• We provide all necessary information in our easy to understand final report
• We participate in EPA DMR-QA Studies annually for the tests conducted in our lab
• We use our in-house culture and local supplier to eliminate organism stress and provide more reliable testing results for our clients

For additional information about Toxicity Testing (or WET) visit EPA's website at Whole Effluent Toxicity (WET) Methods.
Definitions, Acronyms and Abbreviation:
LC50 is the toxicant concentration that would cause death in 50% of the test organisms
IC25 is a point estimate of the toxicant concentration that would cause a 25% reduction in test organism growth or reproduction.
TUa is the reciprocal of the effluent concentration that causes 50 percent of the organisms to die by the end of the acute exposure period. TUa=100/LC50
TUc is the reciprocal of the effluent concentration that causes no observable effect on the test organisms by the end of the chronic exposure period. TUc=100/NOEC
NOEC is the highest tested concentration of toxicant to which organisms are exposed in a full life-cycle or partial life-cycle test that causes no observable adverse effect on the test organism.
LOEC is the lowest concentration of toxicant to which organisms are exposed in a test, which causes statistically significant adverse effects on the test organisms.
PMSD Percent Minimum Significant Difference.
MSD Minimum Significant Different
Reference Links:
www.setac.org
www.nelac-institute.org
www.toxicity.com
www.cfpub.epa.gov
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